Molecular Biophysics

Program manager: Prof.dr. Gijs Wuite

The research in this program focuses on exploring biophysical questions on the level from single molecules to cells. A central question is how protein and DNA structural dynamics are related to their function. The aim is to work with increasingly complex assemblies of biomolecules in order to investigate the emergent properties from these systems. This approach bridges experimental systems biology and single-molecule manipulation techniques. We are also focusing more and more on single-biomolecule dynamics in living cells or organisms. We use a variety of optical techniques such as super-resolution fluorescence microscopy, single-molecule fluorescence spectroscopy, optical tweezers, tethered particle motion, AFM, as well as combinations of these techniques. The data obtained are related to biochemical studies and used for theoretical modeling.