Instant Pathology: Third and second harmonic generation microscopy for the asessment of fresh unprocessed tissue

Laura van Huizen MSc and Professor Marie Louise Groot published an article "Instant Pathology: Third and second harmonic generation microscopy for the assessment of fresh unprocessed tissue" in PHOTONICS Magazine's October 2018 issue.

10/18/2018 | 4:23 PM

One inmoprtant application domain of photonics is medical photonics, where the goal is to make the diagnosis and treatment of diseases faster, more accurate and effective. A promising technique is nonlinear optical microscopy that has evolved as an alternative to conventional single-photon confocal microscopy. It provides several advantgages, such as minimal invasiveness, intrinsic 3D sectioning and resolution high depth penetration, low phototoxicity and it is label-free. One of these nonlinear optical techinques is second and third harmonic generation (THG/SHG) microscopy. Researchers at the Vrije Universiteit Amsterdam in the section Biophotonics and Medical Imaging of Marloes Groot are developing THG/SHG microscopy imaging for clinical application. This article gives an explanation of the imaging technique and discusses some examples of the clinical applications.

Here you can read the complete article.